Tuesday, 2 April 2019

Gastrointestinal Endoscopy in Dogs


Gastrointestinal Endoscopy in Dogs


Introduction
Endoscopy is a Greek word comprising of “Endo” for Inner and “Skopein” to view or observe with a purpose and Fiberoptic endoscopy is a noninvasive technique for evaluating the lumen and mucosa of the gastrointestinal tract of dogs. It is a fundamental method for investigation of the digestive tract, and is important in the diagnosis and prognosis of a variety of gastrointestinal disorders.
It is indicated mainly if history and physical examination of a patient reveals abnormalities in the area of the gastrointestinal tract. In addition to this further investigations like plan and contrast radiography, ultrasonography and functional tests may permit tentative diagnosis. Until recently clinician had been limited in their ability to diagnose GI diseases morphologically because of the need to carry out an exploratory laparotomy in order to obtain biopsy samples and has been largely replaced by endoscopy.


Endoscope’s Structure and Equipment for Endoscopy
According to their structure endoscopes are divided into two groups: rigid endoscopes which are hermetically mounted in a metal tube and have an optical lens system, and flexible fiberscope which have a flexible working part and transmit the imagine via the light fiber optics. Mobility of the distal end of endoscope and its controlled movement in one or two dimensions provides an examination and a biopsy in the necessary place.
Rigid tubes endoscopes (otoscopes, esophagoscopes, rectoscopes, and laparoscopes) are simpler devices that look like metallic tubes through which the cavities are examined by the naked eye. Such devices are used for shallow entering. Flexible endoscopes with fiber optics (fiber endoscopes) differ from the rigid by its flexible working part. It allows the endoscope to go down the bent anatomical channels deep located cavities. To transmit the images the fiber light path with orderly structure of certain fibers is used in flexible endoscopes that helps to prevent image distortion.

Endoscopes Disinfection and Sterilization
Before the examination it is necessary to check the quality of the image, water and air supply, the integrity of the outer hull system. After conducting the examination the biopsy channel of the endoscope should be washed with a Luke-warm water (30-35° C; 2 liters)), followed by washing in running water with neutral detergent. After washing the endoscope should be carefully wiped with a soft cloth. The surface of the flexible tube is wiped with 70% alcohol.
            The valves of the biopsy channel should be taken off and wiped with a sponge and washed by alcohol. After washing the biopsy channel of the endoscope is dried in the air from the insufflator. Biopsy forceps and loops are thoroughly washed in warm water with soap, wiped with alcohol and dried. The final disinfection of fiber endoscopes and instruments is made by plunging them in glutaraldehyde solution, or by gas sterilization with ethylene oxide or formaldehyde. Autoclaving can be used only for tools and parts of the device that cannot be damaged under the influence of heat and pressure.

Preparation of Animal for the Examination
Preparation starts with withholding of food for 36 hours and water for 4 hours prior to examination. A plain Radiograph should be taken prior to examination that may rule out any foreign body obstruction. Endotracheal intubation should be done by tying the endotracheal tube to the mandible instead of maxilla that aids in the passage of the endoscope into the oesophagus. The patient is placed in the left lateral recumbence.

Anesthesia for gastrointestinal endoscopic examination
Animal must be properly restrained during endoscopic examination by giving general anesthesia using mainly intravenous anesthetic products and also preanesthetic like atropine before 30-40 minutes of the examination must be give that reduces the secretion of salivary and other digestive glands and decreases muscle tonicity of gastrointestinal tract. Diazepam is a widely used tranquilizer (2-10 mg intramuscularly).

Endoscopic Procedure
Endoscopy of esophagus, stomach, and duodenum is performed after withholding food for about 36 hours. The endoscope is introduced with the dog in left lateral recumbence. During the examination the presence and character of contents, and the appearance of the mucosa, are noted. In dogs with a body weight of about 15 kg or more, examination of the descending duodenal portion may be possible with this typical endoscope. Biopsies of the various gastric and duodenal areas are taken and the different views are photographed.  
            Endoscopy of colon and rectum is performed under general anesthesia, as mentioned above, after fasting for some 48 hours. Prior to the examination the colon and rectum are cleansed with a water enema. The endoscope is introduced through the anus via a speculum. With insufflation of air, the rectum of colonic canal is followed proximately, if possible to the caeca and ileac region. Examination of the colon and rectum is best performed while the probe is being withdrawn. Biopsies of various areas are taken and different views are photographed.

DIFFERENT ENDOSCCOPIC CHARACTERISTICS OF GIT DISEASE
Endoscopy is useful in the diagnosis of some of the gastrointestinal diseases only and is contraindicated in some conditions like gastro esophageal reflux and mega esophagus.

Esophageal disorders
The endoscopic examination of the esophagus is applied with principal indications of dysphagia and regurgitation. The functional disorders of esophagus are studied with radiographic examination instead of endoscopic examination.
 Foreign body obstruction is not uncommon in dogs particularly in the terrier breeds (rather than cats) because the esophagus is a highly distensible organ. Ulcerative esophagitis is result of esophageal foreign bodies and must be treated as a matter of urgency.
Endoscopic forceps are very helpful in the removal of the foreign body in many animals, provided the entity is minor and restricted in the cervical or cranial thoracic region, and the risks of perforation are low. Esophagitis follows inflammation resulting from various affronts, both acute and chronic. The most common causes of esophagitis are persistent esophageal foreign bodies, ingestion of acidic substances, persistent vomiting. Peptic esophagitis and reflux esophagitis are far less obvious on endoscopy and vary in severity. Erythema or erosions may be seen, particularly in the distal esophagus. In severe cases, the erosions may progress to ulceration and stenosis may result.
Strictures following reflux esophagitis may also present as diffuse or strip-like areas of erosion or generally occur in the form of a regular, concentric narrowing of the lumen,. Successive inflation of balloon catheters introduced via the operating channel of the endoscope helps in the alleviation of the strictures. Strictures occur following ingestion of acidic substances are often far-reaching and carry a very guarded prognosis and it makes the treatment unsuccessful.

Gastric disorders
Indications for gastric endoscopy are chronic vomiting, hematemesis, melena, or severe anemia as a consequence of gastrointestinal bleeding. Acute gastritis is a vague clinical entity associated with loss of integrity of the gastric mucosa. Various etiological agents may be associated with acute gastritis for example, ingestion of a foreign body or caustic materials and infectious disease. Reflux gastritis is usually chronic, but occasionally it may present as an acute syndrome. It follows retrograde movement of irritant biliary and pancreatic secretions which may disrupt the mucosa to cause erosions, particularly on the top of the mucosal folds in the antrums.
 Metabolic disorders and hepatic diseases may result in ischemia of the gastric mucosa. The ischemia is visualized as multiple hemorrhagic mucosal erosions. Chronic gastritis may cause vomiting, weight loss and anorexia. Endoscopic examination and endoscope-guided biopsy have revealed three basic patterns of chronic gastritis. Atrophic type of chronic gastritis is characterized by abnormally pale mucosa with visible sub-mucosal blood vessels and atrophy of the fundic folds.
Nodular type of chronic gastritis is characterized by a papular or nodular reaction, often around the pylorus. Granulomatous type of chronic gastritis is characterized by diffuse or localized hypertrophy of mucosa of antral region which is called chronic hypertrophic pyloric gastropathy or antral pyloric hypertrophy. Pyloric obstruction is a relatively common disorder and results from congenital or acquired stenosis or blockage of the pyloric lumen by a foreign body. CHPG is most common in older, small breed dogs.
It is characterized by the appearance of a diffuse or localized hypertrophy of the anteropyloric mucosa which results in delayed gastric emptying. The clinical signs include chronic vomiting and weight loss. The possibility of visualization of almost pathognomonic hypertrophy may be in the pyloric region, it must be differentiated from neoplastic conditions by biopsy. Congenital pyloric stenosis appears most commonly in Boxers, French Bulldogs, and Boston Terriers, and has an endoscopic appearance that is similar to CHPG. Gastric ulceration appears to be much more common than was supposed before the widespread use of endoscopy.  
Gastric ulceration may reflect a variety of etiologies, such as systemic illness or the use of ulcerogenic drugs (particularly nonsteroidalanti-inflammatory agents). Ulcers that occur as a consequence of these drugs are often small, concentrated in the antropyloric area and appear as linear or lenticular erosions with adjacent, often multiple, superficial bleeding points. Gastric tumors may be either benign or malignant. Many benign gastric tumors are discovered serendipitously at endoscopy. Benign, adenomatous polyps may occur in the antrum and may cause pyloric stenosis. In contrast, leiomyoma’s, typically found in older dogs; appear as ulcerated masses, frequently in the fundus and occasionally at the gastro esophageal junction. Malignant gastric neoplasia in dogs is usually a carcinoma or adenocarcinoma, whereas lymphoma is the most common tumor type in cats. Endoscopically, these gastric neoplasms are pleomorphic.

 Small intestinal disorders
The principal indications for endoscopy of the proximal intestinal tract are chronic vomiting, small intestinal-type diarrhea, and putative malabsorption syndromes. The use of endoscopic catheters to collect duodenal chyme for quantitative bacteriological culture is essential for definitive diagnosis of small intestinal bacterial overgrowth. Endoscopic examination of the intestinal tract may reveal evidence of hyperemia or ulceration, but it is not easy to correlate these signs with disease. In many cases with histopathologically confirmed disease, the endoscopic appearance is normal.
There are no pathognomonic presentations of intestinal disease, and in most cases histopathological examination of biopsy material is necessary to make a diagnosis. Intestinal (duodenal) ulceration is rare in animals. When it does occur, it is generally associated with chronic inflammatory bowel disease or neoplasia, and is often situated close to the pylorus. Intestinal ulcers may perforate, resulting in acute abdominal crisis. Lymphangiectasia presents endoscopically with a multi-focal, pale, papular, almost granular appearance. The mucosa may sometimes have a mucoid appearance due to exudation of lymph nodes.
Intestinal tumors are not readily diagnosed by endoscopy, although occasionally a friable, hemorrhagic mass may be seen. Most commonly a thickening or swelling is noticed and histopathological examination of biopsy samples is necessary to make the diagnosis.

Large intestinal and colorectal disorders
Colonoscopy is necessary if the distal intestinal tract is to be visualized. The principal indications are large bowel diarrhea, tenesmus, and hematochezia . As with the investigation of gastric and small intestinal disorders, it may prove difficult to correlate histopathological evidence of disease with endoscopic appearance, and the clinician must be prepared to take serial biopsy samples from the area under investigation. Colitis may result in discontinuous lesions interspersed between areas of endoscopically normal mucosa, although it is most often diffuse. Lesions may be superficial or extend deep into the sub mucosa. Healing of these deeper lesions causes fibrosis that result in stenosis and formation of pleomorphic pseudo polyp. Chronic inflammation may result in thickened, excessively folded, friable mucosa.

Histological classification of representative lesions, and of apparently normal mucosa, is mandatory. Ileocolic intussusception is not uncommon, particularly in young animals with intestinal motility disorders accompanying diarrhea. It appears endoscopically as a smooth, intraluminal mass. Whip worm infection is associated with Trichuris vulpis, a parasite with a widespread distribution. However, the routine use of gastrointestinal endoparasiticides removes these parasites and thus clinical disease is regional. Trichuriasis is associated with tenesmus, hematochezia, and the presence of colonic inflammation of varying degrees of severity
 Colonic and rectal neoplasia may be benign or malignant. The most common manifestation is benign polyp formation, which has a pleomorphic presentation. These polyps may protrude through the rectum and cause tenesmus and bleeding. Malignant tumors also have a pleomorphic presentation, dependent to some extent on the underlying type and size. Stenosis may result if they are large.

CONCLUSIONS
Endoscopy has proved to be a very valuable and useful tool in the diagnosis of gastrointestinal diseases in the dogs. The use of endoscopy is limited mainly to specialized clinics. It is mainly indicated in dogs with signs of chronic gastrointestinal disease, in which a diagnosis cannot be made with the available diagnostic tools.
  
 References
1. Tams, T. R. Small Animal Endoscopy. St Louis: The CV Mosby Company, 1990: 417.

2. Simpson, K. W. Gastrointestinal endoscopy in the dog. Journal of Small Animal Practice
             1993; 34: 180–188.

3. Sullivan, M., Miller, A. Endoscopy of the oesophagus and stomach in the dog with persistent regurgitation and vomiting. Journal of Small Animal Practice 1985; 26: 369–379.

4. Lecoindre Pan Atlas of gastrointestinal endoscopy in dogs and cats.

5. Edward J Hall, James W. Simpson, David A Williams BSAVA manual of canine and feline gastroenterology.

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